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Image Search Results
Journal: Molecular Cancer
Article Title: Strategies to overcome the main challenges of the use of CRISPR/Cas9 as a replacement for cancer therapy
doi: 10.1186/s12943-021-01487-4
Figure Lengend Snippet: The efficacy of CRISPR-based cancer therapeutics in several different clinical studies
Article Snippet: NCT04211480 , China ,
Techniques: CRISPR, Electroporation, Expressing, Modification, Knock-Out, Mutagenesis, Infection, Genetically Modified, Ex Vivo
Journal: PLoS ONE
Article Title: A Synthetic Model of Human Beta-Thalassemia Erythropoiesis Using CD34+ Cells from Healthy Adult Donors
doi: 10.1371/journal.pone.0068307
Figure Lengend Snippet: (A) Western blot analyses of globin chains were performed using total soluble cytosolic protein (20 µg/lane) from day 14, 18, and 21 cultured erythroblasts. Antibodies against alpha-globin, beta-globin, and gamma-globin were used for comparison. Beta-actin was used as a loading control. (B) Western analysis of the alpha-globin insoluble membrane fraction on culture days 14, 18, and 21 of control and beta-KD cultured erythroblasts. Fractions from equivalent cell number preparations were used in each lane. GPA was used as a loading control for the insoluble membrane proteins. Equivalent film exposure time (one minute) was used for alpha-globin, beta-globin, and gamma-globin membrane comparisons.
Article Snippet: Blots were probed with antibodies against human alpha-globin (Santa Cruz Biotechnology, Santa Cruz, CA), beta-globin (Abnova, Walnut, CA),
Techniques: Western Blot, Cell Culture, Comparison, Control, Membrane