γ globin Search Results


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IQ Products mouse monoclonal antibodies against human γ-globin chain iqp-363-int-4
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WuXi AppTec anti-γ-globin (1:1000)
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BioRay Inc gene-edited autologous hematopoietic stem cells γ-globin expression
The efficacy of CRISPR-based cancer therapeutics in several different clinical studies
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IQ Products mouse monoclonal antibodies against human γ-globin chain
The efficacy of CRISPR-based cancer therapeutics in several different clinical studies
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Abnova gamma-globin
(A) Western blot analyses of globin chains were performed using total soluble cytosolic protein (20 µg/lane) from day 14, 18, and 21 cultured <t>erythroblasts.</t> <t>Antibodies</t> against alpha-globin, beta-globin, and <t>gamma-globin</t> were used for comparison. Beta-actin was used as a loading control. (B) Western analysis of the alpha-globin insoluble membrane fraction on culture days 14, 18, and 21 of control and beta-KD cultured erythroblasts. Fractions from equivalent cell number preparations were used in each lane. GPA was used as a loading control for the insoluble membrane proteins. Equivalent film exposure time (one minute) was used for alpha-globin, beta-globin, and gamma-globin membrane comparisons.
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Amaxa δ-, γ-, or β-globin reporter construct
(A) Western blot analyses of globin chains were performed using total soluble cytosolic protein (20 µg/lane) from day 14, 18, and 21 cultured <t>erythroblasts.</t> <t>Antibodies</t> against alpha-globin, beta-globin, and <t>gamma-globin</t> were used for comparison. Beta-actin was used as a loading control. (B) Western analysis of the alpha-globin insoluble membrane fraction on culture days 14, 18, and 21 of control and beta-KD cultured erythroblasts. Fractions from equivalent cell number preparations were used in each lane. GPA was used as a loading control for the insoluble membrane proteins. Equivalent film exposure time (one minute) was used for alpha-globin, beta-globin, and gamma-globin membrane comparisons.
δ , γ , Or β Globin Reporter Construct, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The efficacy of CRISPR-based cancer therapeutics in several different clinical studies

Journal: Molecular Cancer

Article Title: Strategies to overcome the main challenges of the use of CRISPR/Cas9 as a replacement for cancer therapy

doi: 10.1186/s12943-021-01487-4

Figure Lengend Snippet: The efficacy of CRISPR-based cancer therapeutics in several different clinical studies

Article Snippet: NCT04211480 , China , Bioray Laboratories , Thalassemia Major , 12 , Gene-edited autologous hematopoietic stem cells with γ-globin expression , Unspecified , / , June 1, 2023 , -.

Techniques: CRISPR, Electroporation, Expressing, Modification, Knock-Out, Mutagenesis, Infection, Genetically Modified, Ex Vivo

(A) Western blot analyses of globin chains were performed using total soluble cytosolic protein (20 µg/lane) from day 14, 18, and 21 cultured erythroblasts. Antibodies against alpha-globin, beta-globin, and gamma-globin were used for comparison. Beta-actin was used as a loading control. (B) Western analysis of the alpha-globin insoluble membrane fraction on culture days 14, 18, and 21 of control and beta-KD cultured erythroblasts. Fractions from equivalent cell number preparations were used in each lane. GPA was used as a loading control for the insoluble membrane proteins. Equivalent film exposure time (one minute) was used for alpha-globin, beta-globin, and gamma-globin membrane comparisons.

Journal: PLoS ONE

Article Title: A Synthetic Model of Human Beta-Thalassemia Erythropoiesis Using CD34+ Cells from Healthy Adult Donors

doi: 10.1371/journal.pone.0068307

Figure Lengend Snippet: (A) Western blot analyses of globin chains were performed using total soluble cytosolic protein (20 µg/lane) from day 14, 18, and 21 cultured erythroblasts. Antibodies against alpha-globin, beta-globin, and gamma-globin were used for comparison. Beta-actin was used as a loading control. (B) Western analysis of the alpha-globin insoluble membrane fraction on culture days 14, 18, and 21 of control and beta-KD cultured erythroblasts. Fractions from equivalent cell number preparations were used in each lane. GPA was used as a loading control for the insoluble membrane proteins. Equivalent film exposure time (one minute) was used for alpha-globin, beta-globin, and gamma-globin membrane comparisons.

Article Snippet: Blots were probed with antibodies against human alpha-globin (Santa Cruz Biotechnology, Santa Cruz, CA), beta-globin (Abnova, Walnut, CA), gamma-globin (Abnova), and the appropriate horseradish peroxidase-conjugated secondary antibodies (Santa Cruz Biotechnology).

Techniques: Western Blot, Cell Culture, Comparison, Control, Membrane